Stereo-seq Demo Data
Additional Analysis Figures
Publications
Note: Acknowledges Admera Health “for their invaluable contributions”
Dissociated cells were resuspended in ice-cold PBS containing 0.02% BSA and loaded onto a Chromium Single cell 3′ chip (with an estimated recovery of 6,000 cells per channel) to generate gel beads in emulsion (GEMs). scRNA-seq libraires were prepared with the Chromium Single cell 3′ GEM, Library & Gel Bead Kit v3 (10x Genomics, PN: 1000075). Libraries from different samples were pooled and sequenced by Admera Health on a NovaSeq S4 (Illumina) using 150 × 2 chemistry. Demultiplexing, alignment, barcode and UMI counting and aggregation were performed using Cell Ranger (v3.0 with default settings). This provided us with a feature by count matrix.
Bead Kit v2(10x Genomics, PN:120237). Libraries from different samples were pooled and sequenced by Admera Health on a NovaSeq S4 (Illumina) using 150*2 chemistry.
snRNA-seq libraries were prepared with the Chromium Single cell 3′ GEM, Library & Gel Bead Kit v3 (10x Genomics). Libraries from different samples were pooled and sequenced by Admera Health on a NovaSeq S4 (Illumina).
In order to reach sufficient DNA quantity, the extract was subjected to whole-genome amplification (WGA) with the REPLI-g Single Cell Kit (Qiagen). The WGA product was processed through a Nextera XT library and sequenced on an Illumina HiSeq X machine by Admera Health (South Plainfield, NJ, USA), producing 37,100,964 pairs of 150 bp reads.